Journal: Acta Biochimica et Biophysica Sinica

Article Title: TRIM21 promotes type I interferon by inhibiting the autophagic degradation of STING via p62/SQSTM1 ubiquitination in systemic lupus erythematosus

doi: 10.3724/abbs.2025046

Figure Lengend Snippet: TRIM21 catalyzes K63-linked polyubiquitination of p62/SQSTM1 and suppresses the interaction between p62/SQSTM1 and STING (A) sh-p62 and sh-TRIM21 or OE-TRIM21 were transfected into HEK293T cells, which were subsequently collected and lysed 24 h post-transfection for western blot analysis of p62/SQSTM1, TRIM21, STING, and β-actin. (B) HEK239T cells were transfected with either NC or overexpression TRIM21 (OE-TRIM21) plasmids. Following immunoprecipitation with anti-p62 or normal IgG, the lysates were subjected to immunoblotting using the specified antibodies. (C) HEK293T cells were transfected with NC or OE-TRIM21 plasmids, followed by stimulation with or without 2′3′-cGAMP (2 μg/mL) for 4 h. Subsequently, the cells were fixed, stained with anti-p62 antibody (green) and anti-STING antibody (red), and visualized by confocal microscopy. Scale bar: 50 μm. (D) HEK293T cells were transfected with plasmids encoding Flag-p62, HA-Ub, HA-Ub(K48), HA-Ub(K63), and Myc-TRIM21. Subsequently, after 24 h, the cells were subjected to ubiquitylation assay.

Article Snippet: The HA-Ub (176462), Flag-p62 (204576), HA-Ub (K48) (17604), and HA-Ub (K63) (17606) plasmids were purchased from Addgene (Cambridge, USA).

Techniques: Transfection, Western Blot, Over Expression, Immunoprecipitation, Staining, Confocal Microscopy, Ubiquitin Assay

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: High FIGNL1 expression in human CRC samples. (a) The expression of FIGNL1 in the TCGA database. (b) Immunohistochemical detection of FIGNL1 expression in clinical CRC samples. FIGNL1, fidgetin-like 1; CRC, colorectal cancer.

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 knockdown inhibited Caco-2 cell proliferation, migration, and invasion. (a) Representative blot demonstrating FIGNL1 expression in NCM460, Colo-205, and Caco-2 cells. (b) Bar chart illustrating FIGNL1 mRNA expression following FIGNL1 siRNA treatment in Caco-2 cells. (c) Representative blot depicting FIGNL1 expression following FIGNL1 siRNA treatment in Caco-2 cells. (d) Line chart exhibiting Caco-2 cell proliferation with FIGNL1 siRNA treatment. (e) Cell migration and invasion were examined using transwell assay after FIGNL1 siRNA transfection in Caco-2 cells. Scale bar: 500 µm. * p < 0.05 and ** p < 0.01 versus control siRNA group. Con, control; OD, optical density; FIGNL1, fidgetin-like 1; CRC, colorectal cancer; siRNA, Small interfering RNA.

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: Knockdown, Migration, Expressing, Transwell Assay, Transfection, Control, Small Interfering RNA

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 overexpression promoted Colo-205 cell proliferation, migration, and invasion. (a) Bar chart displaying FIGNL1 mRNA expression following FIGNL1 overexpression lentivirus infection in Colo-205 cells. (b) Representative blot depicting FIGNL1 expression after FIGNL1 overexpression-inducing lentivirus infection in Colo-205 cells. (c) Line chart exhibiting the Colo-205 cell proliefration after FIGNL1 overexpression-inducing lentivirus infection. (d) Cell migration and invasion were assessed using transwell assay after FIGNL1 overexpression-inducing lentivirus infection in Colo-205 cells. Scale bar: 200 µm. * p < 0.05 and ** p < 0.01 versus control overexpression group. FIGNL1, fidgetin-like 1; Con, control; OD, optical density; OE, overexpression.

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: Over Expression, Migration, Expressing, Infection, Transwell Assay, Control

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 promoted CRC progression by activating the P38 pathway in vitro. (a) Representative blots demonstrating ERK/JNK/P38 MAPK expression following FIGNL1 siRNA treatment in Caco-2 cells. (b) Representative blots depicting ERK/JNK/P38 MAPK expression with lentivirus infection-related FIGNL1 overexpression in Colo-205 cells. (c) Line chart illustrating the Colo-205 cell proliferation following sb203580 treatment after FIGNL1 overexpression and control lentivirus infection. (d) Cell migration and invasion were examined by transwell assay after FIGNL1 overexpression-inducing lentivirus infection and sb203580 treatment in Colo-205 cells. Scale bar: 200 µm. * p < 0.05 versus control overexpression group. # p < 0.05 versus FIGNL1 overexpression group. FIGNL1, Fidgetin-like 1; Con, control; OD, optical density, OE, overexpression; siRNA, Small interfering RNA

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: In Vitro, Expressing, Infection, Over Expression, Control, Migration, Transwell Assay, Small Interfering RNA

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 activated P38 phosphorylation via SPIDR. (a) Prediction of proteins interacting with FIGNL1. (b) Gene enrichment was attained through gene ontology analysis. (c) The collective scores of various indicators regarding the interaction between FIGNL1 and the targeted proteins. (d) Representative blots depicting the binding between FIGNL1 and SPIDR. (e) Representative blots demonstrating P38 phosphorylation in FIGNL1-overexpression Colo-205 cells after SPIDR siRNA treatment. FIGNL1, Fidgetin-like 1; Con, control; OE, overexpression; IP, immunoprecipitation.

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: Phospho-proteomics, Binding Assay, Over Expression, Control, Immunoprecipitation

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 interacting with SPIDR and facilitating CRC cell proliferation, migration, and invasion by activating the P38 pathway. FIGNL1, Fidgetin-like 1; CRC, colorectal cancer.

Article Snippet: Details of primer antibodies used in this study are as follows: FIGNL1 (17604-1-AP, Proteintech, Wuhan, China), GADPH (60004-1-Ig, Proteintech, Wuhan, China), p-JNK (80024-1-AP, Proteintech, Wuhan, China), T-JNK (24164-1-AP, Proteintech, Wuhan, China), p-ERK (28733-1-AP, Proteintech, Wuhan, China), T-ERK (11257-1-AP, Proteintech, Wuhan, China), p-P38 (28796-1-AP, Proteintech, Wuhan, China), T-P38 (14064-1-AP, Proteintech, Wuhan, China), SPIDR (HPA041582, Sigma-Aldrich, Missouri, USA).

Techniques: Migration

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: High FIGNL1 expression in human CRC samples. (a) The expression of FIGNL1 in the TCGA database. (b) Immunohistochemical detection of FIGNL1 expression in clinical CRC samples. FIGNL1, fidgetin-like 1; CRC, colorectal cancer.

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Immunohistochemical staining

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 knockdown inhibited Caco-2 cell proliferation, migration, and invasion. (a) Representative blot demonstrating FIGNL1 expression in NCM460, Colo-205, and Caco-2 cells. (b) Bar chart illustrating FIGNL1 mRNA expression following FIGNL1 siRNA treatment in Caco-2 cells. (c) Representative blot depicting FIGNL1 expression following FIGNL1 siRNA treatment in Caco-2 cells. (d) Line chart exhibiting Caco-2 cell proliferation with FIGNL1 siRNA treatment. (e) Cell migration and invasion were examined using transwell assay after FIGNL1 siRNA transfection in Caco-2 cells. Scale bar: 500 µm. * p < 0.05 and ** p < 0.01 versus control siRNA group. Con, control; OD, optical density; FIGNL1, fidgetin-like 1; CRC, colorectal cancer; siRNA, Small interfering RNA.

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: Knockdown, Migration, Expressing, Transwell Assay, Transfection, Control, Small Interfering RNA

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 overexpression promoted Colo-205 cell proliferation, migration, and invasion. (a) Bar chart displaying FIGNL1 mRNA expression following FIGNL1 overexpression lentivirus infection in Colo-205 cells. (b) Representative blot depicting FIGNL1 expression after FIGNL1 overexpression-inducing lentivirus infection in Colo-205 cells. (c) Line chart exhibiting the Colo-205 cell proliefration after FIGNL1 overexpression-inducing lentivirus infection. (d) Cell migration and invasion were assessed using transwell assay after FIGNL1 overexpression-inducing lentivirus infection in Colo-205 cells. Scale bar: 200 µm. * p < 0.05 and ** p < 0.01 versus control overexpression group. FIGNL1, fidgetin-like 1; Con, control; OD, optical density; OE, overexpression.

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: Over Expression, Migration, Expressing, Infection, Transwell Assay, Control

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 promoted CRC progression by activating the P38 pathway in vitro. (a) Representative blots demonstrating ERK/JNK/P38 MAPK expression following FIGNL1 siRNA treatment in Caco-2 cells. (b) Representative blots depicting ERK/JNK/P38 MAPK expression with lentivirus infection-related FIGNL1 overexpression in Colo-205 cells. (c) Line chart illustrating the Colo-205 cell proliferation following sb203580 treatment after FIGNL1 overexpression and control lentivirus infection. (d) Cell migration and invasion were examined by transwell assay after FIGNL1 overexpression-inducing lentivirus infection and sb203580 treatment in Colo-205 cells. Scale bar: 200 µm. * p < 0.05 versus control overexpression group. # p < 0.05 versus FIGNL1 overexpression group. FIGNL1, Fidgetin-like 1; Con, control; OD, optical density, OE, overexpression; siRNA, Small interfering RNA

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: In Vitro, Expressing, Infection, Over Expression, Control, Migration, Transwell Assay, Small Interfering RNA

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 activated P38 phosphorylation via SPIDR. (a) Prediction of proteins interacting with FIGNL1. (b) Gene enrichment was attained through gene ontology analysis. (c) The collective scores of various indicators regarding the interaction between FIGNL1 and the targeted proteins. (d) Representative blots depicting the binding between FIGNL1 and SPIDR. (e) Representative blots demonstrating P38 phosphorylation in FIGNL1-overexpression Colo-205 cells after SPIDR siRNA treatment. FIGNL1, Fidgetin-like 1; Con, control; OE, overexpression; IP, immunoprecipitation.

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: Phospho-proteomics, Binding Assay, Over Expression, Control, Immunoprecipitation

Journal: Balkan Medical Journal

Article Title: Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments

doi: 10.4274/balkanmedj.galenos.2024.2024-7-9

Figure Lengend Snippet: FIGNL1 interacting with SPIDR and facilitating CRC cell proliferation, migration, and invasion by activating the P38 pathway. FIGNL1, Fidgetin-like 1; CRC, colorectal cancer.

Article Snippet: Following embedding, slicing, dewaxing, and hydration, the slides were stained with the primary antibody against FIGNL1 (1:200, 17604-1-AP, Proteintech, Wuhan, China).

Techniques: Migration